Heat Injury of Bacillus subtilis Spores at Ultrahigh Temperatures1
نویسندگان
چکیده
منابع مشابه
Thermal inactivation characteristics of Bacillus subtilis spores at ultrahigh temperatures.
The thermal inactivation characteristics of Bacillus subtilis A spores suspended in skim milk with the use of large-scale ultrahigh temperature (UHT) processing equipment were investigated in terms of survival as measured with two plating media. Data on survival immediately after UHT treatments were recorded in temperature-survivor curves, time-survivor curves, and decimal reduction time (DRT) ...
متن کاملHow moist heat kills spores of Bacillus subtilis.
Populations of Bacillus subtilis spores in which 90 to 99.9% of the spores had been killed by moist heat gave only two fractions on equilibrium density gradient centrifugation: a fraction comprised of less dense spores that had lost their dipicolinic acid (DPA), undergone significant protein denaturation, and were all dead and a fraction with the same higher density as that of unheated spores. ...
متن کاملHeat Resistance and Population Stability of Lyophilized Bacillus subtilis Spores.
Bacillus subtilis 5230 spores were lyophilized in 0.067 M phosphate buffer and stored at 2 to 8 degrees C for 9 to 27 months. The lyophilized spores were reconstituted with buffer or 0.9% saline, and the heat resistance was determined in a thermoresistometer. Lyophilization had no effect on the heat resistance of the spores but did result in a slight decrease in population (</=0.3-logarithm red...
متن کاملHeat shock proteins do not influence wet heat resistance of Bacillus subtilis spores.
Spores of Bacillus subtilis are significantly more resistant to wet heat than are their vegetative cell counterparts. Analysis of the effects of mutations in and the expression of fusions of a coding gene for a thermostable beta-galactosidase to a number of heat shock genes has shown that heat shock proteins play no significant role in the wet heat resistance of B. subtilis spores.
متن کاملResistance of Bacillus subtilis Spores to Inactivation
A statistical evaluation of viable count procedures utilized for obtaining treatment survival curve data for Bacillus subtilis NCTC 8236 spores is described. Within the various recovery conditions tested, incubation on nutrient agar containing 1% dextrose for 48 hr at 37 C was found to promote the highest count of viable spores surviving a variety of bactericidal treatments involving gamma irra...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1965
ISSN: 0003-6919
DOI: 10.1128/aem.13.6.858-864.1965